diff --git a/ilastik/1.4.1/Dockerfile b/ilastik/1.4.1/Dockerfile
new file mode 100644
index 00000000..3a69cb54
--- /dev/null
+++ b/ilastik/1.4.1/Dockerfile
@@ -0,0 +1,55 @@
+FROM ubuntu:22.04 
+
+LABEL base_image="ubuntu:22.04"
+LABEL version="1"
+LABEL software="ilastik"
+LABEL software.version="1.4.1"
+LABEL about.summary="the interactive learning and segmentation toolkit"
+LABEL about.home="https://ilastik.org"
+LABEL about.license="GPL-2.0-or-later"
+LABEL about.license_file="https://github.com/ilastik/ilastik/blob/main/LICENSE"
+LABEL about.documentation="https://ilastik.org/documentation/index.html"
+LABEL extra.identifiers.biotools=ilastik
+
+
+MAINTAINER Yi Sun <sunyi000@gmail.com>
+
+
+ARG DEBIAN_FRONTEND="noninteractive"
+ARG ILASTIK_VERSION="1.4.1"
+
+ENV LANG=en_US.UTF-8 \
+    LC_ALL=en_US.UTF-8 \
+    LANGUAGE=en_US:en  \
+    QT_XCB_NO_MITSHM=1 \
+    LAZYFLOW_THREADS=2 \
+    LAZYFLOW_TOTAL_RAM_MB=32000 \
+    NUMBA_CACHE_DIR=/tmp/numba_cache \
+    BIOIMAGEIO_CACHE_PATH="/tmp/pybio_cache" \
+    PATH="/opt/ilastik-$ILASTIK_VERSION-Linux:$PATH"
+
+RUN apt-get update -qq && \
+    apt-get install -y  \
+            apt-utils \
+            locales \
+            build-essential \
+            libglu1-mesa-dev \
+            qtcreator \
+            libgl1-mesa-glx \
+            nautilus \
+            xdg-utils \
+            wget && \
+    apt-get clean && \
+    rm -rf /var/lib/apt/lists/* 
+
+
+RUN sed -i '/en_US.UTF-8/s/^# //g' /etc/locale.gen && \
+    locale-gen
+
+WORKDIR /opt/
+RUN wget https://files.ilastik.org/ilastik-1.4.1-Linux.tar.bz2 && \
+    tar xjf ilastik-1.*-Linux.tar.bz2 && \
+    rm -f ilastik-1.*-Linux.tar.bz2
+
+
+#ENTRYPOINT["run_ilastik.sh"]
diff --git a/ilastik/1.4.1/test-cmds.txt b/ilastik/1.4.1/test-cmds.txt
new file mode 100644
index 00000000..debf9d52
--- /dev/null
+++ b/ilastik/1.4.1/test-cmds.txt
@@ -0,0 +1 @@
+/opt/ilastik-1.4.1-Linux/run_ilastik.sh --headless --version