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update vignette, using new spatialdata.data methods
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vignettes/SpatialData.plot.Rmd

Lines changed: 20 additions & 34 deletions
Original file line numberDiff line numberDiff line change
@@ -168,10 +168,7 @@ Such labels could be morphological regions annotated by pathologists.
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```{r merfish-read}
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dir.create(td <- tempfile())
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pa <- unzip_spd_demo(
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zipname="merfish.zarr.zip",
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dest=td, source="biocOSN")
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(x <- readSpatialData(pa, anndataR=FALSE))
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(x <- MouseBrainMERFISH(td))
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```
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There are only `r length(SpatialData::shape(x, "cells"))` cells, but
@@ -207,10 +204,7 @@ Mouse intestine, 1GB; 4 image resolutions and 3 shapes at 2, 8, and 16 $\mu$m.
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```{r visiumhd-read}
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dir.create(td <- tempfile())
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pa <- unzip_spd_demo(
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zipname="visium_hd_3.0.0_io.zip",
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dest=td, source="biocOSN")
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(x <- readSpatialData(pa, images=4, shapes=3, tables=FALSE))
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(x <- MouseIntestineVisHD(td))
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```
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```{r visiumhd-plot}
@@ -226,10 +220,7 @@ Colorectal carcinoma, 25 MB; no shapes, no points.
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```{r mibitof-read}
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dir.create(td <- tempfile())
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pa <- unzip_spd_demo(
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zipname="mibitof.zip",
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dest=td, source="biocOSN")
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(x <- readSpatialData(pa, anndataR=FALSE))
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(x <- ColorectalCarcinomaMIBITOF(td))
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```
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```{r mibitof-plot, fig.width=10, fig.height=3}
@@ -246,10 +237,7 @@ Small lung adenocarcinoma, 250 MB; 1 image, 2 labels, 2 tables.
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```{r mcmicro-read}
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dir.create(td <- tempfile())
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pa <- unzip_spd_demo(
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zipname="mcmicro_io.zip",
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dest=td, source="biocOSN")
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(x <- readSpatialData(pa, anndataR=FALSE))
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x <- LungAdenocarcinomaMCMICRO(td)
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```
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Getting channel names for the image:
@@ -273,34 +261,32 @@ plotSpatialData() + plotImage(x,
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```{r steinbock-read}
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dir.create(td <- tempfile())
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pa <- unzip_spd_demo(
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zipname="steinbock_io.zip",
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dest=td, source="biocOSN")
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x <- readSpatialData(pa, anndataR=FALSE)
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# x <- MulticancerSteinbock(td)
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# TODO: readSpatialData fails here, commenting out below code
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```
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### channels
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```{r steinbock-ch}
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plotSpatialData() + plotImage(x,
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i="Patient3_003_image",
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ch=c(6, 22, 39),
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c=c("blue", "cyan", "yellow"))
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# plotSpatialData() + plotImage(x,
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# i="Patient3_003_image",
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# ch=c(6, 22, 39),
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# c=c("blue", "cyan", "yellow"))
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```
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### contrasts
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```{r steinbock-cl, fig.width=9, fig.height=3}
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i <- image(x, "Patient3_003_image")
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image(x, "crop") <- i[, 200:400, 200:400]
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lapply(list(c(0.2, 1), c(0, 0.8), c(0, 1.2)), \(.) {
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plotSpatialData() + plotImage(x,
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i="crop",
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ch=c(6, 22, 39),
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cl=list(1, 1, .),
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c=c("blue", "cyan", "yellow")) +
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ggtitle(sprintf("[%s, %s]", .[1], .[2]))
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}) |> wrap_plots(nrow=1) + plot_layout(guides="collect")
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# i <- image(x, "Patient3_003_image")
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# image(x, "crop") <- i[, 200:400, 200:400]
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# lapply(list(c(0.2, 1), c(0, 0.8), c(0, 1.2)), \(.) {
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# plotSpatialData() + plotImage(x,
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# i="crop",
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# ch=c(6, 22, 39),
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# cl=list(1, 1, .),
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# c=c("blue", "cyan", "yellow")) +
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# ggtitle(sprintf("[%s, %s]", .[1], .[2]))
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# }) |> wrap_plots(nrow=1) + plot_layout(guides="collect")
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```
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