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fixing more check problems
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DESCRIPTION

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DESeq2
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VignetteBuilder: knitr
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RoxygenNote: 7.2.3
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Date: 2021-06-23
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Date: 2023-04-03
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Encoding: UTF-8
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biocViews:
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LinkingTo: Rcpp

R/primersearch.R

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#'
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#' A pair of primers are aligned against a set of sequences.
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#' The location of the best hits, quality of match, and predicted amplicons are returned.
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#' Requires the EMBOSS tool kit (\url{http://emboss.sourceforge.net/}) to be installed.
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#' Requires the EMBOSS tool kit (\url{https://emboss.sourceforge.net/}) to be installed.
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#'
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#' It can be confusing how the primer sequence relates to the binding sites on a
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#' reference database sequence. A simplified diagram can help. For example, if
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#' \code{\link{taxmap}} object with two tables is returned: a table with
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#' information for each predicted amplicon, quality of match, and predicted
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#' amplicons, and a table with per-taxon amplification statistics. Requires the
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#' EMBOSS tool kit (\url{http://emboss.sourceforge.net/}) to be installed.
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#' EMBOSS tool kit (\url{https://emboss.sourceforge.net/}) to be installed.
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#'
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#' It can be confusing how the primer sequence relates to the binding sites on a
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#' reference database sequence. A simplified diagram can help. For example, if

README.Rmd

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```
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[![Build Status](https://travis-ci.com/grunwaldlab/metacoder.png?branch=master)](https://travis-ci.com/grunwaldlab/metacoder?branch=master) [![codecov.io](https://codecov.io/github/grunwaldlab/metacoder/coverage.svg?branch=master)](https://codecov.io/github/grunwaldlab/metacoder?branch=master)
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[![Downloads from Rstudio mirror per month](http://cranlogs.r-pkg.org/badges/metacoder)](https://www.r-pkg.org:443/pkg/metacoder)
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[![Downloads from Rstudio mirror](http://cranlogs.r-pkg.org/badges/grand-total/metacoder)](https://www.r-pkg.org:443/pkg/metacoder)
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[![CRAN version](http://www.r-pkg.org/badges/version/metacoder)](https://cran.r-project.org/package=metacoder)

README.md

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<!-- README.md is generated from README.Rmd. Please edit that file -->
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[![Build
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Status](https://travis-ci.com/grunwaldlab/metacoder.png?branch=master)](https://travis-ci.com/grunwaldlab/metacoder?branch=master)
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[![codecov.io](https://codecov.io/github/grunwaldlab/metacoder/coverage.svg?branch=master)](https://codecov.io/github/grunwaldlab/metacoder?branch=master)
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[![Downloads from Rstudio mirror per
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month](http://cranlogs.r-pkg.org/badges/metacoder)](https://www.r-pkg.org:443/pkg/metacoder)
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[![Downloads from Rstudio
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microbiome bioinformatics on data in the `taxmap` format defined by the
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`taxa` package, such as:
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- Summing read counts/abundance per taxon
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- Converting counts to proportions and rarefaction of counts using
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`vegan`
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- Comparing the abundance (or other characteristics) of groups of
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samples (e.g., experimental treatments) per taxon
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- Combining data for groups of samples
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- Simulated PCR, via EMBOSS primersearch, for testing primer
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specificity and coverage of taxonomic groups
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- Converting common microbiome formats for data and reference
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databases into the objects defined by the `taxa` package.
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- Converting to and from the `phyloseq` format and the `taxa` format
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- Summing read counts/abundance per taxon
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- Converting counts to proportions and rarefaction of counts using
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`vegan`
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- Comparing the abundance (or other characteristics) of groups of
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samples (e.g., experimental treatments) per taxon
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- Combining data for groups of samples
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- Simulated PCR, via EMBOSS primersearch, for testing primer specificity
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and coverage of taxonomic groups
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- Converting common microbiome formats for data and reference databases
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into the objects defined by the `taxa` package.
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- Converting to and from the `phyloseq` format and the `taxa` format
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## Installation
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Metacoder is under active development and many new features are planned.
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Some improvements that are being explored include:
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- Barcoding gap analysis and associated plotting functions
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- A function to aid in retrieving appropriate sequence data from NCBI
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for *in silico* PCR from whole genome sequences
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- Graphing of different node shapes in heat trees, possibly including
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pie graphs or [PhyloPics](http://phylopic.org/).
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- Adding the ability to plot specific edge lengths in the heat trees
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so they can be used for phylogenetic trees.
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- Adding more data import and export functions to make parsing and
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writing common formats easier.
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- Barcoding gap analysis and associated plotting functions
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- A function to aid in retrieving appropriate sequence data from NCBI
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for *in silico* PCR from whole genome sequences
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- Graphing of different node shapes in heat trees, possibly including
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pie graphs or [PhyloPics](http://phylopic.org/).
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- Adding the ability to plot specific edge lengths in the heat trees so
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they can be used for phylogenetic trees.
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- Adding more data import and export functions to make parsing and
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writing common formats easier.
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To see the details of what is being worked on, check out the
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[issues](https://github.com/grunwaldlab/metacoder/issues) tab of the

cran-comments.md

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## Test environments and check results
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### local: Pop!_OS 20.04 LTS, R 4.0.3
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### local: Pop!_OS 22.04 LTS, R 4.2.2
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0 errors | 0 warnings | 0 notes
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0 errors | 0 warnings | 1 notes
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### travis-ci: release
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❯ checking dependencies in R code ... NOTE
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Namespace in Imports field not imported from: ‘R6’
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All declared Imports should be used.
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I am not sure why this note exists. I am using `R6::` in about 10 places in the package.
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All of the classes in the package are R6 classes, so it is definitely used.
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0 errors | 0 warnings | 0 notes
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### Rhub: Ubuntu Linux 20.04.1 LTS, R-release, GCC
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### Rhub: Windows
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0 errors | 0 warnings | 0 notes
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0 errors | 0 warnings | 2 notes
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### All testing platforms using R devel (rhub, winbuilder)
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* checking dependencies in R code ... NOTE
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Namespace in Imports field not imported from: 'R6'
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All declared Imports should be used.
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I am not sure why this note exists. I am using `R6::` in about 10 places in the package.
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All of the classes in the package are R6 classes, so it is definitely used.
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Some bioconductor packages in Suggests seem to not be available for R 4.2. I get the following error on R devel builds:
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* checking for detritus in the temp directory ... NOTE
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Found the following files/directories:
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'lastMiKTeXException'
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Error: Bioconductor does not yet build and check packages for R version 4.2
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Seems to be a bug with Rhub:
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https://github.com/r-hub/rhub/issues/503
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However, all of the bioconductor packages are optional and not needed for most functionality.
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## Downstream dependencies

man/primersearch.Rd

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man/primersearch_raw.Rd

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