diff --git a/Lib/test/test_bytes.py b/Lib/test/test_bytes.py
index 21be61e4fec720..44b16c7d91e996 100644
--- a/Lib/test/test_bytes.py
+++ b/Lib/test/test_bytes.py
@@ -2092,6 +2092,19 @@ def make_case():
         with self.assertRaises(BufferError):
             ba.rsplit(evil)
 
+    def test_hex_use_after_free(self):
+        # Prevent UAF in bytearray.hex(sep) with re-entrant sep.__len__.
+        # Regression test for https://github.com/python/cpython/issues/143195.
+        ba = bytearray(b'\xAA')
+
+        class S(bytes):
+            def __len__(self):
+                ba.clear()
+                return 1
+
+        self.assertRaises(BufferError, ba.hex, S(b':'))
+
+
 class AssortedBytesTest(unittest.TestCase):
     #
     # Test various combinations of bytes and bytearray
diff --git a/Lib/test/test_memoryview.py b/Lib/test/test_memoryview.py
index 1bd58eb6408833..51b107103f6836 100644
--- a/Lib/test/test_memoryview.py
+++ b/Lib/test/test_memoryview.py
@@ -442,6 +442,20 @@ def test_issue22668(self):
         self.assertEqual(c.format, "H")
         self.assertEqual(d.format, "H")
 
+    def test_hex_use_after_free(self):
+        # Prevent UAF in memoryview.hex(sep) with re-entrant sep.__len__.
+        # Regression test for https://github.com/python/cpython/issues/143195.
+        ba = bytearray(b'A' * 1024)
+        mv = memoryview(ba)
+
+        class S(bytes):
+            def __len__(self):
+                mv.release()
+                ba.clear()
+                return 1
+
+        self.assertRaises(BufferError, mv.hex, S(b':'))
+
 
 # Variations on source objects for the buffer: bytes-like objects, then arrays
 # with itemsize > 1.
diff --git a/Misc/NEWS.d/next/Core_and_Builtins/2025-12-27-10-14-26.gh-issue-143195.MNldfr.rst b/Misc/NEWS.d/next/Core_and_Builtins/2025-12-27-10-14-26.gh-issue-143195.MNldfr.rst
new file mode 100644
index 00000000000000..66dc5e22f0ab23
--- /dev/null
+++ b/Misc/NEWS.d/next/Core_and_Builtins/2025-12-27-10-14-26.gh-issue-143195.MNldfr.rst
@@ -0,0 +1,3 @@
+Fix use-after-free crashes in :meth:`bytearray.hex` and :meth:`memoryview.hex`
+when the separator's :meth:`~object.__len__` mutates the original object.
+Patch by Bénédikt Tran.
diff --git a/Objects/bytearrayobject.c b/Objects/bytearrayobject.c
index 338c71ad38f7aa..8a454aa48a0930 100644
--- a/Objects/bytearrayobject.c
+++ b/Objects/bytearrayobject.c
@@ -2664,7 +2664,13 @@ bytearray_hex_impl(PyByteArrayObject *self, PyObject *sep, int bytes_per_sep)
 {
     char* argbuf = PyByteArray_AS_STRING(self);
     Py_ssize_t arglen = PyByteArray_GET_SIZE(self);
-    return _Py_strhex_with_sep(argbuf, arglen, sep, bytes_per_sep);
+    // Prevent 'self' from being freed if computing len(sep) mutates 'self'
+    // in _Py_strhex_with_sep().
+    // See: https://github.com/python/cpython/issues/143195.
+    self->ob_exports++;
+    PyObject *res = _Py_strhex_with_sep(argbuf, arglen, sep, bytes_per_sep);
+    self->ob_exports--;
+    return res;
 }
 
 static PyObject *
diff --git a/Objects/memoryobject.c b/Objects/memoryobject.c
index f1232f389210ea..2fd1d784b92ec8 100644
--- a/Objects/memoryobject.c
+++ b/Objects/memoryobject.c
@@ -2349,7 +2349,13 @@ memoryview_hex_impl(PyMemoryViewObject *self, PyObject *sep,
     CHECK_RELEASED(self);
 
     if (MV_C_CONTIGUOUS(self->flags)) {
-        return _Py_strhex_with_sep(src->buf, src->len, sep, bytes_per_sep);
+        // Prevent 'self' from being freed if computing len(sep) mutates 'self'
+        // in _Py_strhex_with_sep().
+        // See: https://github.com/python/cpython/issues/143195.
+        self->exports++;
+        PyObject *ret = _Py_strhex_with_sep(src->buf, src->len, sep, bytes_per_sep);
+        self->exports--;
+        return ret;
     }
 
     PyBytesWriter *writer = PyBytesWriter_Create(src->len);