|
| 1 | +test_that("get_count_datasets works", { |
| 2 | + # Use testthat edition 3 |
| 3 | + local_edition(3) |
| 4 | + |
| 5 | + # Generate test SE |
| 6 | + se <- SummarizedExperiment::SummarizedExperiment( |
| 7 | + assays = list( |
| 8 | + mat1 = matrix(seq_len(9), nrow = 3), |
| 9 | + mat2 = matrix(seq(10, 18), nrow = 3), |
| 10 | + mat3 = matrix(seq(19, 27), nrow = 3) |
| 11 | + ) |
| 12 | + ) |
| 13 | + rownames(se) <- paste0("G", seq_len(3)) |
| 14 | + colnames(se) <- paste0("S", seq_len(3)) |
| 15 | + SummarizedExperiment::assays(se) <- lapply( |
| 16 | + SummarizedExperiment::assays(se), |
| 17 | + function(x) { |
| 18 | + rownames(x) <- rownames(se) |
| 19 | + colnames(x) <- colnames(se) |
| 20 | + x |
| 21 | + } |
| 22 | + ) |
| 23 | + |
| 24 | + # Check that dimnames are as expected |
| 25 | + expect_equal(rownames(se), rownames(assay(se, "mat1", withDimnames = FALSE))) |
| 26 | + expect_equal(rownames(se), rownames(assay(se, "mat2", withDimnames = FALSE))) |
| 27 | + expect_equal(rownames(se), rownames(assay(se, "mat3", withDimnames = FALSE))) |
| 28 | + expect_equal(colnames(se), colnames(assay(se, "mat1", withDimnames = FALSE))) |
| 29 | + expect_equal(colnames(se), colnames(assay(se, "mat2", withDimnames = FALSE))) |
| 30 | + expect_equal(colnames(se), colnames(assay(se, "mat3", withDimnames = FALSE))) |
| 31 | + # Check that matrix values are as expected |
| 32 | + expect_equal(assay(se, "mat1")[, 1], c(1, 2, 3), ignore_attr = TRUE) |
| 33 | + expect_equal(assay(se, "mat2")[, 2], c(13, 14, 15), ignore_attr = TRUE) |
| 34 | + expect_equal(assay(se, "mat3")[, 3], c(25, 26, 27), ignore_attr = TRUE) |
| 35 | + |
| 36 | + # SE and all assays have the same dimnames |
| 37 | + cds <- get_count_datasets(se) |
| 38 | + expect_s3_class(cds, "tbl_df") |
| 39 | + expect_equal(nrow(cds), 9L) |
| 40 | + expect_equal(ncol(cds), 5L) |
| 41 | + expect_named(cds, c(".feature", ".sample", "mat1", "mat2", "mat3")) |
| 42 | + expect_equal(cds$.feature, rep(paste0("G", seq_len(3)), 3)) |
| 43 | + expect_equal(cds$.sample, rep(paste0("S", seq_len(3)), each = 3)) |
| 44 | + expect_equal(cds$mat1, seq(1, 9)) |
| 45 | + expect_equal(cds$mat2, seq(10, 18)) |
| 46 | + expect_equal(cds$mat3, seq(19, 27)) |
| 47 | + |
| 48 | + # SE does not have dimnames, all assays have the same |
| 49 | + se1 <- se |
| 50 | + rownames(se1) <- colnames(se1) <- NULL |
| 51 | + expect_equal(colnames(assay(se1, "mat1", withDimnames = FALSE)), paste0("S", seq_len(3))) |
| 52 | + expect_equal(rownames(assay(se1, "mat1", withDimnames = FALSE)), paste0("G", seq_len(3))) |
| 53 | + expect_null(colnames(se1)) |
| 54 | + expect_null(rownames(se1)) |
| 55 | + expect_warning(expect_warning(cds <- get_count_datasets(se1), "have column names, but the SummarizedExperiment does not"), "have row names, but the SummarizedExperiment does not") |
| 56 | + expect_s3_class(cds, "tbl_df") |
| 57 | + expect_equal(nrow(cds), 9L) |
| 58 | + expect_equal(ncol(cds), 5L) |
| 59 | + expect_named(cds, c(".feature", ".sample", "mat1", "mat2", "mat3")) |
| 60 | + expect_equal(cds$.feature, rep(paste0("G", seq_len(3)), 3)) |
| 61 | + expect_equal(cds$.sample, rep(paste0("S", seq_len(3)), each = 3)) |
| 62 | + expect_equal(cds$mat1, seq(1, 9)) |
| 63 | + expect_equal(cds$mat2, seq(10, 18)) |
| 64 | + expect_equal(cds$mat3, seq(19, 27)) |
| 65 | + |
| 66 | + # SE does not have dimnames, two assays have the same, third assay does not have -> error |
| 67 | + se1 <- se |
| 68 | + rownames(se1) <- colnames(se1) <- NULL |
| 69 | + rownames(assay(se1, "mat2", withDimnames = FALSE)) <- |
| 70 | + colnames(assay(se1, "mat2", withDimnames = FALSE)) <- NULL |
| 71 | + expect_error(get_count_datasets(se1), "at least one of the assays in your SummarizedExperiment have column names") |
| 72 | + |
| 73 | + # SE does not have dimnames, assays have the same but in different order |
| 74 | + se1 <- se |
| 75 | + rownames(se1) <- colnames(se1) <- NULL |
| 76 | + colnames(assay(se1, "mat2", withDimnames = FALSE)) <- c("S2", "S3", "S1") |
| 77 | + rownames(assay(se1, "mat3", withDimnames = FALSE)) <- c("G2", "G3", "G1") |
| 78 | + expect_warning(expect_warning(cds <- get_count_datasets(se1), "have column names, but the SummarizedExperiment does not"), "have row names, but the SummarizedExperiment does not") |
| 79 | + expect_s3_class(cds, "tbl_df") |
| 80 | + expect_equal(nrow(cds), 9L) |
| 81 | + expect_equal(ncol(cds), 5L) |
| 82 | + expect_named(cds, c(".feature", ".sample", "mat1", "mat2", "mat3")) |
| 83 | + expect_equal(cds$.feature, rep(paste0("G", seq_len(3)), 3)) |
| 84 | + expect_equal(cds$.sample, rep(paste0("S", seq_len(3)), each = 3)) |
| 85 | + expect_equal(cds$mat1, seq(1, 9)) |
| 86 | + expect_equal(cds$mat2, c(16, 17, 18, 10, 11, 12, 13, 14, 15)) |
| 87 | + expect_equal(cds$mat3, c(21, 19, 20, 24, 22, 23, 27, 25, 26)) |
| 88 | + |
| 89 | + # SE does not have dimnames, assays have nonoverlapping dimnames -> error |
| 90 | + se1 <- se |
| 91 | + rownames(se1) <- colnames(se1) <- NULL |
| 92 | + rownames(assay(se1, "mat2", withDimnames = FALSE)) <- paste0("A", seq_len(3)) |
| 93 | + expect_error(get_count_datasets(se1), "at least one of the assays in your SummarizedExperiment have row names") |
| 94 | + |
| 95 | + se1 <- se |
| 96 | + rownames(se1) <- colnames(se1) <- NULL |
| 97 | + colnames(assay(se1, "mat2", withDimnames = FALSE)) <- paste0("A", seq_len(3)) |
| 98 | + expect_error(get_count_datasets(se1), "at least one of the assays in your SummarizedExperiment have column names") |
| 99 | + |
| 100 | + # Neither SE nor assays have column names |
| 101 | + se1 <- se |
| 102 | + colnames(se1) <- NULL |
| 103 | + colnames(assay(se1, "mat1", withDimnames = FALSE)) <- NULL |
| 104 | + colnames(assay(se1, "mat2", withDimnames = FALSE)) <- NULL |
| 105 | + colnames(assay(se1, "mat3", withDimnames = FALSE)) <- NULL |
| 106 | + cds <- get_count_datasets(se1) |
| 107 | + expect_s3_class(cds, "tbl_df") |
| 108 | + expect_equal(nrow(cds), 9L) |
| 109 | + expect_equal(ncol(cds), 5L) |
| 110 | + expect_named(cds, c(".feature", ".sample", "mat1", "mat2", "mat3")) |
| 111 | + expect_equal(cds$.feature, rep(paste0("G", seq_len(3)), 3)) |
| 112 | + expect_equal(cds$.sample, rep(as.character(seq_len(3)), each = 3)) |
| 113 | + expect_equal(cds$mat1, seq(1, 9)) |
| 114 | + expect_equal(cds$mat2, seq(10, 18)) |
| 115 | + expect_equal(cds$mat3, seq(19, 27)) |
| 116 | + |
| 117 | + # Neither SE nor assays have row names |
| 118 | + se1 <- se |
| 119 | + rownames(se1) <- NULL |
| 120 | + rownames(assay(se1, "mat1", withDimnames = FALSE)) <- NULL |
| 121 | + rownames(assay(se1, "mat2", withDimnames = FALSE)) <- NULL |
| 122 | + rownames(assay(se1, "mat3", withDimnames = FALSE)) <- NULL |
| 123 | + cds <- get_count_datasets(se1) |
| 124 | + expect_s3_class(cds, "tbl_df") |
| 125 | + expect_equal(nrow(cds), 9L) |
| 126 | + expect_equal(ncol(cds), 5L) |
| 127 | + expect_named(cds, c(".feature", ".sample", "mat1", "mat2", "mat3")) |
| 128 | + expect_equal(cds$.feature, rep(as.character(seq_len(3)), 3)) |
| 129 | + expect_equal(cds$.sample, rep(paste0("S", seq_len(3)), each = 3)) |
| 130 | + expect_equal(cds$mat1, seq(1, 9)) |
| 131 | + expect_equal(cds$mat2, seq(10, 18)) |
| 132 | + expect_equal(cds$mat3, seq(19, 27)) |
| 133 | + |
| 134 | + # Neither SE nor assays have any dimnames |
| 135 | + se1 <- se |
| 136 | + rownames(se1) <- NULL |
| 137 | + colnames(se1) <- NULL |
| 138 | + rownames(assay(se1, "mat1", withDimnames = FALSE)) <- NULL |
| 139 | + colnames(assay(se1, "mat1", withDimnames = FALSE)) <- NULL |
| 140 | + rownames(assay(se1, "mat2", withDimnames = FALSE)) <- NULL |
| 141 | + colnames(assay(se1, "mat2", withDimnames = FALSE)) <- NULL |
| 142 | + rownames(assay(se1, "mat3", withDimnames = FALSE)) <- NULL |
| 143 | + colnames(assay(se1, "mat3", withDimnames = FALSE)) <- NULL |
| 144 | + cds <- get_count_datasets(se1) |
| 145 | + expect_s3_class(cds, "tbl_df") |
| 146 | + expect_equal(nrow(cds), 9L) |
| 147 | + expect_equal(ncol(cds), 5L) |
| 148 | + expect_named(cds, c(".feature", ".sample", "mat1", "mat2", "mat3")) |
| 149 | + expect_equal(cds$.feature, rep(as.character(seq_len(3)), 3)) |
| 150 | + expect_equal(cds$.sample, rep(as.character(seq_len(3)), each = 3)) |
| 151 | + expect_equal(cds$mat1, seq(1, 9)) |
| 152 | + expect_equal(cds$mat2, seq(10, 18)) |
| 153 | + expect_equal(cds$mat3, seq(19, 27)) |
| 154 | + |
| 155 | + # SE has dimnames, assays have the same dimnames but not overlapping with those of the SE |
| 156 | + se1 <- se |
| 157 | + rownames(se1) <- colnames(se1) <- paste0("A", seq_len(3)) |
| 158 | + expect_error(get_count_datasets(se1), "don't agree with the column names of the SummarizedExperiment") |
| 159 | + |
| 160 | + se1 <- se |
| 161 | + rownames(se1) <- paste0("A", seq_len(3)) |
| 162 | + expect_error(get_count_datasets(se1), "don't agree with the row names of the SummarizedExperiment") |
| 163 | + |
| 164 | + # SE has dimnames, none of the assays have |
| 165 | + se1 <- se |
| 166 | + rownames(assay(se1, "mat1", withDimnames = FALSE)) <- NULL |
| 167 | + colnames(assay(se1, "mat1", withDimnames = FALSE)) <- NULL |
| 168 | + rownames(assay(se1, "mat2", withDimnames = FALSE)) <- NULL |
| 169 | + colnames(assay(se1, "mat2", withDimnames = FALSE)) <- NULL |
| 170 | + rownames(assay(se1, "mat3", withDimnames = FALSE)) <- NULL |
| 171 | + colnames(assay(se1, "mat3", withDimnames = FALSE)) <- NULL |
| 172 | + cds <- get_count_datasets(se1) |
| 173 | + expect_s3_class(cds, "tbl_df") |
| 174 | + expect_equal(nrow(cds), 9L) |
| 175 | + expect_equal(ncol(cds), 5L) |
| 176 | + expect_named(cds, c(".feature", ".sample", "mat1", "mat2", "mat3")) |
| 177 | + expect_equal(cds$.feature, rep(paste0("G", seq_len(3)), 3)) |
| 178 | + expect_equal(cds$.sample, rep(paste0("S", seq_len(3)), each = 3)) |
| 179 | + expect_equal(cds$mat1, seq(1, 9)) |
| 180 | + expect_equal(cds$mat2, seq(10, 18)) |
| 181 | + expect_equal(cds$mat3, seq(19, 27)) |
| 182 | + |
| 183 | + # Unnamed assay(s) |
| 184 | + # se1 <- SummarizedExperiment::SummarizedExperiment( |
| 185 | + # assays = list( |
| 186 | + # matrix(seq_len(9), nrow = 3), |
| 187 | + # mat2 = matrix(seq(10, 18), nrow = 3), |
| 188 | + # matrix(seq(19, 27), nrow = 3) |
| 189 | + # ) |
| 190 | + # ) |
| 191 | + # expect_error(cds <- get_count_datasets(se1), "assays must be named") |
| 192 | + # |
| 193 | + # se1 <- SummarizedExperiment::SummarizedExperiment( |
| 194 | + # assays = list( |
| 195 | + # matrix(seq_len(9), nrow = 3), |
| 196 | + # matrix(seq(10, 18), nrow = 3), |
| 197 | + # matrix(seq(19, 27), nrow = 3) |
| 198 | + # ) |
| 199 | + # ) |
| 200 | + # expect_error(cds <- get_count_datasets(se1), "assays must be named") |
| 201 | +}) |
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