@@ -167,8 +167,7 @@ In this example data, we do not have a `label` for the `shape` polygons.
167167Such labels could be morphological regions annotated by pathologists.
168168
169169``` {r merfish-read}
170- dir.create(td <- tempfile())
171- (x <- MouseBrainMERFISH(td))
170+ (x <- MouseBrainMERFISH())
172171```
173172
174173There are only ` r length(SpatialData::shape(x, "cells")) ` cells, but
@@ -203,8 +202,7 @@ plotSpatialData() + plotImage(x) + bb | plotSpatialData() + plotImage(y)
203202Mouse intestine, 1GB; 4 image resolutions and 3 shapes at 2, 8, and 16 $\mu$m.
204203
205204``` {r visiumhd-read}
206- dir.create(td <- tempfile())
207- (x <- MouseIntestineVisHD(td))
205+ (x <- MouseIntestineVisHD())
208206```
209207
210208``` {r visiumhd-plot}
@@ -219,8 +217,7 @@ plotSpatialData() + plotImage(x) + bb | plotSpatialData() + plotImage(y)
219217Colorectal carcinoma, 25 MB; no shapes, no points.
220218
221219``` {r mibitof-read}
222- dir.create(td <- tempfile())
223- (x <- ColorectalCarcinomaMIBITOF(td))
220+ (x <- ColorectalCarcinomaMIBITOF())
224221```
225222
226223``` {r mibitof-plot, fig.width=10, fig.height=3}
@@ -236,8 +233,7 @@ wrap_plots(nrow=1, lapply(seq(3), \(.)
236233Small lung adenocarcinoma, 250 MB; 1 image, 2 labels, 2 tables.
237234
238235``` {r mcmicro-read}
239- dir.create(td <- tempfile())
240- x <- LungAdenocarcinomaMCMICRO(td)
236+ x <- LungAdenocarcinomaMCMICRO()
241237```
242238
243239Getting channel names for the image:
@@ -260,33 +256,31 @@ plotSpatialData() + plotImage(x,
2602564 different cancers (SCCHN, BCC, NSCLC, CRC), 820 MB; 14 images, 14 labels, 1 table.
261257
262258``` {r steinbock-read}
263- dir.create(td <- tempfile())
264- # x <- MulticancerSteinbock(td)
265- # TODO: readSpatialData fails here, commenting out below code
259+ x <- MulticancerSteinbock()
266260```
267261
268262### channels
269263
270264``` {r steinbock-ch}
271- # plotSpatialData() + plotImage(x,
272- # i="Patient3_003_image",
273- # ch=c(6, 22, 39),
274- # c=c("blue", "cyan", "yellow"))
265+ plotSpatialData() + plotImage(x,
266+ i="Patient3_003_image",
267+ ch=c(6, 22, 39),
268+ c=c("blue", "cyan", "yellow"))
275269```
276270
277271### contrasts
278272
279273``` {r steinbock-cl, fig.width=9, fig.height=3}
280- # i <- image(x, "Patient3_003_image")
281- # image(x, "crop") <- i[, 200:400, 200:400]
282- # lapply(list(c(0.2, 1), c(0, 0.8), c(0, 1.2)), \(.) {
283- # plotSpatialData() + plotImage(x,
284- # i="crop",
285- # ch=c(6, 22, 39),
286- # cl=list(1, 1, .),
287- # c=c("blue", "cyan", "yellow")) +
288- # ggtitle(sprintf("[%s, %s]", .[1], .[2]))
289- # }) |> wrap_plots(nrow=1) + plot_layout(guides="collect")
274+ i <- image(x, "Patient3_003_image")
275+ image(x, "crop") <- i[, 200:400, 200:400]
276+ lapply(list(c(0.2, 1), c(0, 0.8), c(0, 1.2)), \(.) {
277+ plotSpatialData() + plotImage(x,
278+ i="crop",
279+ ch=c(6, 22, 39),
280+ cl=list(1, 1, .),
281+ c=c("blue", "cyan", "yellow")) +
282+ ggtitle(sprintf("[%s, %s]", .[1], .[2]))
283+ }) |> wrap_plots(nrow=1) + plot_layout(guides="collect")
290284```
291285
292286# Masking
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