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Introduction

multiomicGWAS is a flexible GWAS pipeline leveraging GWASpoly to perform genome-wide association analyses across ploidy levels from 2 to 8. The pipeline can integrate secondary traits and host-associated metagenome/microbiome data as covariate, and it can also allow each microbial taxon to be treated as an independent phenotypic trait for GWAS. Relationship matrices are computed automatically: genomic relationship matrices (GRM) using AGHmatrix and microbiome/metagenome kernels using the Aitchison method for compositional data, enabling streamlined multi-omic association analyses.

For questions, bugs, and suggestions, please contact bolukolu@utk.edu.

How to run multiomicGWAS

Download the <run_parameters_multiomicGWAS.R>, edit parameters and run the pipeline.

Description of parameters

Dependencies

  • GWASpoly
  • AGHmatrix
  • qqplotr
  • ggplot2
  • dplyr
  • data.table
  • stringr
  • heatmaply
  • ppcor
  • zoo
  • GGally
  • reshape2
  • compositions
  • sommer
  • mice
  • qvalue

Select Article Referencing GBSapp

Acknowledgment

Troubleshooting

Versioning

Versioning will follow major.minor.patch semantic versioning format.

License

Apache License Version 2.0

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GWAS pipeline with multi-omics integration

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